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Approximately 647,000 people die from heart disease each year in the US, which equates to 1 in every 4 deaths. In the years 2014 and 2015, heart disease cost the United States about $219 billion each year, which includes costs from health care services, medicines, and lost productivity due to death. Finding therapeutic interventions in heart disease not only will improve the patients’ life quality but also lighten the economic burden on the society.

In 2017, scientists from Case Western Reserve University, who published a study in the Journal of Molecular and Cellular Cardiology, found nicotinamide mononucleotide (NMN) supplementation promotes the health of the cell’s powerhouse, the mitochondria, and prevents heart failure. Previous studies have associated mitochondrial dysfunction with heart failure, and researchers have recognized reductions in nicotinamide adenine dinucleotide (NAD+) levels in mitochondrial dysfunction.

While NAD+ is an essential molecule for metabolism and mitochondrial health, NAD+ levels fall in the body with age. Healthy NAD+ levels support various molecular functions in the body, including proteins called sirtuins. These proteins play essential roles in cellular mechanisms by removing molecular tags from other proteins in the cell so they can function to maintain mitochondrial health.

In their experimentation for the study, the researchers used mice deficient in a molecule called KLF4, which regulates mitochondrial health. KLF4-deficient mice have higher sensitivity to heart pressure overload-induced heart failure due to their dysfunctional mitochondria. The underlying cellular mechanisms explaining these effects remained unknown, and the team of researchers wanted to know why.

On the mitochondrial proteins of KLF4-deficient mice, the team found significantly higher amounts of molecular tags, which sirtuins, namely Sirt3, should remove. In heart-health-impaired mice, the scientists observed reduced levels of Sirt3 along with low NAD+ concentrations in the hearts of the animals. Lower NAD+ levels could inhibit the function of NAD+-dependent Sirt3, leaving higher levels of molecular tags on mitochondrial proteins that may inhibit mitochondrial health.

To raise the levels of NAD+ content in the mice, the researchers injected the animals with NMN, a NAD+-boosting molecule. Following the administration of NMN, the activation of inflammation genes in KLF4-deficient mice dropped. Overactivation of  inflammation genes have shown to play a causal role in heart disease, according to previous studies. The NMN treated mice had normal cardiac responses to induced blood pressure overload along with reduced expression of inflammation genes.

(Zhang et al., 2017 | Journal of Cellular Cardiology) NMN reduces the expression of genes associated with inflammation following blood pressure-induced damage through restriction of a component of the heart called the aorta.

A high mortality rate exists in KLF4-deficient mice, and NMN administration conferred a 100% survival rate in these mice following the induction of high blood pressure in the heart. These results demonstrated NMN prevents heart failure in these mice sensitive to blood pressure-induced heart failure. The prevention of heart failure and mitochondrial health also has a correlation. KLF4-deficient mice typically have degenerated and fragmented mitochondria, however, NMN treatment almost completely restored the structure of the mitochondria.

(Zhang et al., 2017 | Journal of Cellular Cardiology) NMN restores mitochondrial structure, called “ultrastructure” in KLF4-deficient mice. The top row of images shows mitochondria in these mice following blood pressure-induced damage, and the bottom row of images shows KLF4-deficient mitochondria following this damage and NMN treatment. The separated images on the left show cardiac mitochondria of normal mice.

“Short-term administration of NMN, a precursor of NAD+, preserved mitochondrial homeostasis and rescued heart function from pressure overload-induced heart failure,” stated the scientists of the study. They also noted that NMN may have preserved mitochondrial function and prevented heart failure by boosting NAD+ levels, improving sirtuin function. Increased sirtuin function led to reduced molecular tagging, which improved the activation of  proteins in mitochondria important for cellular energy production and health. Following their findings,NMN could be an effective therapy for heart failure.