Even with improvements in medical treatments in the last 20 years, there have not been reductions in the mortality rate from intracerebral hemorrhage.  In the Chinese population, intracerebral hemorrhage stroke accounts for approximately 33% of all strokes, compared to 12% of strokes in Caucasians.3  The mortality rate a month after an onset stroke is about 40%.1,3,4  Surviving patients often have to live with long-term mental and physical disorders.2,3

Previous studies indicate NMN injections significantly reduce mortality from stroke and promote functional recovery from stroke in a mouse model.  The current study seeks to examine effects of NMN in a mouse model of intracerebral hemorrhage stroke through collagenase-induced intracerebral hemorrhage mouse model.  The scientists examine body weight recovery, neurological function, and cell stress following stroke to measure the effects of NMN.

Shu et al. (2020)

The results of the study indicate administration of NMN after intracerebral hemorrhage in mice improves neurological function.  Mice with NMN administration walk across a beam better 24 hours after intracerebral hemorrhage compared to mice without. Ability to walk across a beam indicates neurological function recovery.

Shu et al. (2020)

Researchers find  long-term benefits of NMN administration in recovering body weight after intracerebral hemorrhage in mice.  The red line in the graph above represents the body weight, as a percentage of full body weight, over seven days of NMN administration, following an intracerebral hemorrhage.  The black line represents body weight percentage over seven days following intracerebral hemorrhage of mice not given NMN. Across all seven days, the group of mice administered NMN has a higher full body weight percentage compared to the mice not given NMN.

Shu et al. (2020)

Results indicate NMN administration can restore cells so they are not as stressed following intracerebral hemorrhage stroke.  The scientists stain the neurons with NOX-1, which stains more cells with more reactive oxygen present. Reactive oxygen indicates cellular stress.  As the figure above indicates, cellular stress increases following intracerebral hemorrhage; but NMN administration improves the stressful cellular conditions in mice.

The scientists obtain further results indicating cell death of neurons increases following intracerebral hemorrhage.  Cell death of neurons decreases with NMN administration following intracerebral hemorrhage in mice. The team uses a stain called TUNEL staining to measure cell death.  In the area where blood pools between the brain and the brain’s outermost covering from the stroke, the scientists observe a higher concentration of TUNEL staining.

The study presents interesting results, because the past 20 years of medical advancement have not led to a decreased mortality rate from intracerebral hemorrhage.  This indicates researchers will need to develop new strategies to treat intracerebral hemorrhage strokes. Positive effects of NMN following intracerebral hemorrhage were found in mice in this study; NMN improves damage of intracerebral hemorrhage to mice tissues.